Application Cases

Experiment Name: Synergistic Enhancement of Sonodynamic and Photodynamic Therapy for Deep Tissue

Research Direction: Synergistic Enhancement of Sonodynamic and Photodynamic Therapy

Experiment Content: The ATA-4315 high-voltage power amplifier is used to drive an ultrasonic transducer, generating a high-intensity focused acoustic field. The acoustic pressure gradient guides the scattered light to focus, thereby enhancing the penetration depth and light energy transfer efficiency of the 660 nm therapeutic laser in a biomimetic tissue model.

Testing Equipment: ATA-4315 high-voltage power amplifier, UV spectrophotometer, etc.

Figure 1: Experimental Setup

Experiment Process:

1. Photosensitizer and Probe System Construction: Methylene blue (MB) is used as the photosensitizer (PS), and 1,3-diphenylisobenzofuran (DPBF) is used as the singlet oxygen (102) detection probe. The specific and irreversible reaction between DPBF and 102 generates 1,2-diphenylbenzofuran, establishing a model for assessing photodynamic efficiency. DPBF solution is added to the MB solution, and the photodynamic reaction is triggered by 660 nm laser irradiation, with real-time monitoring of the absorbance changes at 420 nm.

2. Setting Up Control and Experimental Groups: The control group does not use ultrasound, while the experimental group uses the ATA-4315 high-voltage power amplifier to amplify the driving signal, generating focused ultrasound within the medium. Scattering phantoms with thicknesses of 5 mm and 10 mm are used to simulate the light transmission characteristics of biological tissues at different depths.

3. Environmental Interference Control and Data Acquisition: Baseline absorbance data are recorded in a natural environment (without laser irradiation) to exclude environmental interference. A spectrophotometer is used to quantitatively analyze the DPBF emission spectra (0-3 min) under four experimental conditions (no laser irradiation, normal irradiation, ultrasound with 5 mm/10 mm scattering phantoms).

Test Results:

1. Singlet Oxygen Generation Efficiency Comparison: Control group (no acoustic field): 5 mm scattering phantom results in a 52.7% decrease in absorbance, and 10 mm scattering phantom results in a 54.2% decrease in absorbance. Experimental group (with acoustic field): 5 mm scattering phantom shows a 66.3% decrease in absorbance under the acoustic field, indicating that sonodynamic and photodynamic synergy significantly enhances light energy utilization.

2. Verification of the Mechanism of Synergistic Enhancement by Acoustic Field: The acoustic radiation force generated by the acoustic field acts on the scattering medium, causing the scattered light to focus, thereby increasing the light energy absorbed by MB and increasing the singlet oxygen production rate by 25.7% compared to the control group (5 mm scattering medium). In a 10 mm thick scattering medium, the acoustic field regulation still maintains a 54.2% decrease in absorbance, proving that the penetration depth is doubled.

Power Amplifier Recommendation: ATA-4315 High-Voltage Power Amplifier

Figure: Specifications of the ATA-4315 High-Voltage Power Amplifier