Application Cases

【Overview】
In 2024, a research team from Central South University published a paper titled "Size-Specific Liposomal Drugs Based on Cavitation Technology for Selective Pharmacokinetics and Pharmacodynamics Studies". In their study, they used the Aigtek ATA-2031 high-voltage amplifier to build an experimental system for cavitation-assisted microfluidic devices. This method helps precisely adjust the particle size distribution of liposomal drugs without altering the flow rate ratio (FRR). Liposomal drugs with different size distributions prepared using this method exhibited differential drug distribution and antitumor efficacy in tumor-bearing animals and melanoma patient-derived organoid models, demonstrating the significant potential of this method in modulating pharmacodynamics and pharmacokinetics.

Experiment Name: Application of ATA-2031 High-Voltage Amplifier in Acoustofluidic Devices for Cavitation-Assisted Microfluidics

Research Direction: Cavitation-Assisted Microfluidic Mixing

Experimental Equipment: ATA-2031 High-Voltage Amplifier, Signal Generator, Micro Syringe Pump, etc.

Experimental Content:
This study constructed a cavitation-assisted microfluidic device and conducted research on its theoretical model and mechanism, design and fabrication, and its application in synthesizing liposomal drugs. A new method for precisely controlling the particle size distribution of liposomal drugs based on cavitation-assisted microfluidic devices was developed.

Experimental Procedure:
The signal generator and power amplifier were connected, and the positive and negative terminals of the power amplifier were connected to the corresponding wires of the cavitation-assisted microfluidic device. Using a micro syringe pump, buffer solution and lipid ethanol solution were injected into the buffer inlet and lipid ethanol inlet, respectively, at specific flow rates. Simultaneously, the signal generator and power amplifier were turned on, and different input voltages were set. As the lipid organic solution and buffer mixed in the fluid-focusing mixing unit, the organic solvent rapidly dissolved into the aqueous phase, causing a sharp decrease in its concentration and leading to the rapid formation of liposomes from the lipid molecules in the buffer.

Additionally, the cavitation-assisted microfluidic device could generate cavitation effects within the channel, further improving mixing efficiency and influencing the initial state of lipid self-assembly. In the experiments, three conditions with FRRs of 3, 4, and 5 were tested, with input peak-to-peak voltages of 100 V, 200 V, and 300 V, respectively.

Experimental Results:
A systematic optimization study of the process parameters of the cavitation-assisted microfluidic device was conducted. By adjusting the voltage intensity of the ultrasonic transducer, it was observed that liposome formation was closely related to the cavitation intensity. Appropriately increasing the voltage significantly enhanced the cavitation effect within the microchannel, thereby improving microfluidic mixing efficiency.

Furthermore, experiments verified that the cavitation-assisted microfluidic device was effective for synthesizing liposomes with different excipients. In terms of ultrasonic transducer frequency, this study also found that appropriate frequency settings were crucial for optimizing cavitation efficiency. This part of the research not only provides a scientific basis for the application of cavitation-assisted microfluidic devices in liposomal drug synthesis but also lays a foundation for subsequent drug preparation and application optimization.

Advantages of Aigtek Amplifiers in This Application:

1. High voltage output and wide power coverage – Enables fine control of cavitation intensity, supporting precise regulation of liposome particle size distribution.

2. Wide bandwidth and high slew rate – Accurately matches the operating frequency of the transducer, ensuring high energy conversion efficiency of high-frequency acoustic waves.

3. Low distortion and real-time monitoring capability – Ensures high consistency in repeated experiments with multiple parameters, improving result reliability.

Recommended Product: ATA-2031 High-Voltage Amplifier

Figure: ATA-2031 High-Voltage Amplifier Specifications and Parameters