Application Cases

Experiment Name: Digital Microfluidics-Based Real-Time Coagulation Detection Technology

Research Direction:
By using voltage to drive blood droplets, changes in the blood droplets during motion are detected. In this experiment, different voltage groups are set to control the motion of blood droplets and determine the appropriate voltage for driving.

Test Equipment:
ATA-7020 high-voltage amplifier, signal source, etc.

Figure 1: Experimental Framework Diagram

Experimental Procedure:

1. A host computer control program sends signals to the control module, which regulates the switching of high and low voltage levels on the PCB. A high-voltage power supply directly provides the required voltage to the PCB electrodes.

2. The ATA-7020 amplifier supplies different high-voltage groups. In this experiment, voltages ranging from 150V to 300V are used to verify droplet actuation.

3. Different voltage groups are set to control the motion of blood droplets. The droplet speed is observed to determine the appropriate motion speed for subsequent experimental operations.

Experimental Results:

Figure 2: Experimental Results

1. Droplet actuation experiments verified that among the four voltage groups tested (200V, 230V, 260V, and 290V), voltages below 200V failed to actuate droplets effectively, while the 290V group risked droplet breakdown and device damage. Based on experimental requirements, 230V was selected as the driving voltage for subsequent experiments.

2. After droplet validation, blood droplet experiments were conducted. By detecting changes in the length and speed of blood droplets during motion, the collected data were processed to analyze parameters such as RT (coagulation reaction time) and clot deformation ability. These parameters were then correlated with clinical gold standard measurements.

3. Correlation analysis revealed strong associations between experimental coagulation reaction time and clot deformation ability with clinical coagulation markers, specifically prothrombin time and fibrinogen concentration. This holds promise for developing a coagulation detection device for point-of-care diagnostics.

Recommended High-Voltage Amplifier: ATA-7020

Figure: ATA-7020 High-Voltage Amplifier Specifications

This document is compiled and published by Aigtek. For more case studies and detailed product information, please continue to follow us. Xi'an Aigtek has become a large-scale instrument and equipment supplier in the industry with an extensive product line, and all demo units are available for free trial.