Application Cases

Experiment Name: Application of Power Amplifier in Promoting Maturation of Myocardial Tissue via Magnetic Field

Experimental Content:
A alternating magnetic field stimulation system was constructed to apply long-term magnetic field stimulation to cardiomyocytes cultured on oriented electromagnetic composite hydrogel scaffolds. The experimental results verified that the combined effect of the alternating magnetic field and the oriented electromagnetic composite hydrogel scaffold promotes the maturation of myocardial tissue, and the oriented structure of the electromagnetic composite hydrogel scaffold can guide the alignment of myocardial tissue. The study investigates the effects of oriented electromagnetic composite scaffolds and alternating magnetic field stimulation on the growth of cardiomyocytes.

Experimental Equipment: Solenoid coil, signal generator, ATA-304 power amplifier, well plates, cells, etc.

Experimental Procedure:

Construction of the Alternating Magnetic Field Stimulation System:
Based on the requirements for magnetic field strength and the size of the uniform magnetic field region needed for magnetic field stimulation, a solenoid coil was designed and self-made. The coil frame was fabricated using organic glass, and enameled wire with a diameter of 0.67 mm was tightly wound to form 146 turns. The coil diameter and length were both 110 mm.

Physical Diagram of the Solenoid Coil

The alternating magnetic field stimulation system consists of three parts: the self-made solenoid coil, a signal generator, and a power amplifier. A schematic diagram of the system is shown in the figure. A sinusoidal AC signal was output by the signal generator (RIGOL DG1022U), amplified by the power amplifier (Aigtek ATA-304), and used to drive the coil to generate an alternating magnetic field.

Schematic Diagram of the Alternating Electric Field Stimulation System

The self-built alternating magnetic field stimulation system was used to apply long-term alternating magnetic field stimulation to cardiomyocytes cultured on electromagnetic composite scaffolds, studying the combined effect of the oriented electromagnetic composite hydrogel and the magnetic field on cardiomyocyte maturation. The beating of the cardiomyocytes was characterized using a dynamic video analysis program.

Experimental Results:
The experimental results showed that cardiomyocytes cultured on oriented electromagnetic composite scaffolds exhibited faster beating rates, better beating synchronicity and orientation, and a contraction direction consistent with the oriented structure of the electromagnetic composite scaffold under alternating magnetic field stimulation. This indicates that cardiomyocytes demonstrate stronger beating ability under the combined action of the oriented electromagnetic composite hydrogel and the alternating magnetic field, and the oriented structure of the oriented electromagnetic composite scaffold can guide the contraction direction of the cardiomyocytes.

Figure: ATA-304C Power Amplifier Specifications and Parameters